It is a sample collection and processing technology used for cytology testing, specifically the liquid-based Pap test, developed by BD (Becton Dickinson). This technology is designed to improve the quality of cytology samples, increase diagnostic accuracy, and may also include automated solutions to facilitate sample processing.
Main Features.
- Sample Collection:
- The sample is taken using a collection device (such as a cervical brush or spatula).
- The device head is detached and placed in a vial containing a specific preservative fluid (SurePath preservative fluid).
- This method allows a greater number of representative cells of the cervix to be preserved.
- Transport and Conservation:
- The composition of the SurePath vial medium has a composition that keeps the cells suspended and well preserved morphologically, preventing cell decomposition during transport to the laboratory.
- The preservative medium also inactivates potential pathogens and facilitates the safe handling of the sample.
- Automated Processing:
- In the laboratory, the sample is processed using automated equipment such as the BD PrepStain™ System.
- This system separates cells from mucus, blood, and other contaminants by centrifugation and a cell stratification process.
- A uniform monolayer of cells is prepared on a slide, which facilitates a more accurate cytological evaluation.
- Diagnostic Advantages:
- Greater sensitivity and specificity in the detection of intraepithelial cervical lesions (compared to the conventional Pap smear).
- Reduction of false negatives due to better cell representation and less interference from blood or inflammation.
- The remaining sample in the vial can be used for complementary tests such as detection of HPV (Human Papillomavirus) by molecular techniques (PCR or hybrid capture).
- Compatibility:
- Compatible with Papanicolaou staining techniques.
- Allows integration with automated analysis and image digitization for computer-assisted evaluation.
Clinical Applications
- Gynecological cytology: main use in cervical cancer screening.
- Non-gynecological cytology: such as urine, sputum, body fluids (pleural, peritoneal), brushings, and washings.
